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td enzyme  (Illumina Inc)


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    Structured Review

    Illumina Inc td enzyme
    Td Enzyme, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 984 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/td+enzyme/Illumina+Tagment+DNA+Enzyme+and+Buffer+Small+Kit/pmc12915527-164-26-29
    Average 97 stars, based on 984 article reviews
    td enzyme - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    other:

    Article Title: DNA-guided transcription factor cooperativity shapes face and limb mesenchyme
    Article Snippet: Lysate was centrifuged for 10 min at 500g at 4°C and then resuspended in transposition buffer (25 ul TD buffer and 2.5 ul TD enzyme (Illumina, 20034197), 16.5 ul PBS, 0.01% digitonin, 0.1% Tween-20, and water up to 50 ul).

    Centrifugation:

    Article Title: IFNγ-induced memory in human macrophages is sustained by the durability of cytokine signaling itself.
    Article Snippet: .. Nuclei were pelleted by centrifugation at 500 g for 10 min and suspended in transposase reaction mixture consisting of 25 μl 2× TD Buffer, 2.5 μl TD enzyme (20034197; Illumina), and 22.5 μl water. ..

    Article Title: IFNγ-induced memory in human macrophages is sustained by the durability of cytokine signaling itself
    Article Snippet: .. Nuclei were pelleted by centrifugation at 500 g for 10 min and suspended in transposase reaction mixture consisting of 25 μl 2× TD Buffer, 2.5 μl TD enzyme (20034197; Illumina), and 22.5 μl water. ..

    Article Title: Genetic investigation of fibromuscular dysplasia identifies risk loci and shared genetics with common cardiovascular diseases
    Article Snippet: Briefly, cells were washed in PBS and incubated for 3 min in 50 μL Resuspension Buffer (10 mM Tris-HCL, pH 7.4, 10 mM NaCl, 3 mM MgCl 2 , all from Sigma-Aldrich, Saint-Louis, MI, USA) containing 0.1% Tween 20 (Sigma-Aldrich, Saint-Louis, MI, USA), 0.1% IGEPAL CA-630 (Sigma-Aldrich, Saint-Louis, MI, USA) and 0.01% Digitonin (Thermo Fisher Scientific, Waltham, MA, USA), before 1 mL Resuspension Buffer with 0.1%Tween 20 was added to the cells. .. After centrifugation, isolated nuclei were resuspended in Transposition Buffer (50 μL, made from 16.5 μL PBS, 5 μL H 2 O, 0.5 μL Tween 20 (10%), 0.5 μL Digitonin 1%, 25 μL 2× TD Buffer and 2.5 μL TD Enzyme, last two from Illumina, San Diego, CA, USA). .. Then, the transposition reaction was purified with MinElute PCR Purification Kit (QIAGEN, Hilden, Germany).

    Article Title: IFNγ-induced memory in human macrophages is not sustained by epigenetic changes but the durability of the cytokine itself
    Article Snippet: .. Nuclei were pelleted by centrifugation at 500g for 10 minutes and suspected in transposase reaction mixture consisting of 25μL 2x TD Buffer, 2.5μL TD enzyme (Illumina, 20034197), and 22.5μL water. ..

    Isolation:

    Article Title: Genetic investigation of fibromuscular dysplasia identifies risk loci and shared genetics with common cardiovascular diseases
    Article Snippet: Briefly, cells were washed in PBS and incubated for 3 min in 50 μL Resuspension Buffer (10 mM Tris-HCL, pH 7.4, 10 mM NaCl, 3 mM MgCl 2 , all from Sigma-Aldrich, Saint-Louis, MI, USA) containing 0.1% Tween 20 (Sigma-Aldrich, Saint-Louis, MI, USA), 0.1% IGEPAL CA-630 (Sigma-Aldrich, Saint-Louis, MI, USA) and 0.01% Digitonin (Thermo Fisher Scientific, Waltham, MA, USA), before 1 mL Resuspension Buffer with 0.1%Tween 20 was added to the cells. .. After centrifugation, isolated nuclei were resuspended in Transposition Buffer (50 μL, made from 16.5 μL PBS, 5 μL H 2 O, 0.5 μL Tween 20 (10%), 0.5 μL Digitonin 1%, 25 μL 2× TD Buffer and 2.5 μL TD Enzyme, last two from Illumina, San Diego, CA, USA). .. Then, the transposition reaction was purified with MinElute PCR Purification Kit (QIAGEN, Hilden, Germany).

    Lysis:

    Article Title: Gene regulation by convergent promoters
    Article Snippet: .. After 3 min, the lysis buffer was washed out with 1 ml resuspension buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl 2 , 0.1% Tween-20), cells were pelleted (500 g , 4 °C, 10 min) and resuspended in 50 μl transposition mixture (25 μl 2× TD buffer (Illumina), 2.5 μl TD enzyme (Illumina), 16.5 μl PBS, 0.5 μl 1% digitonin, 0.5 μl 10% Tween-20, 5 μl H 2 O). .. After incubation (37 °C, 1,000 rpm, 30 min), DNA was purified and Illumina adapters were added.



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